Genemark DNA Clean-Up & Extraction Kit

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DP03P-Genemark Plus Gel Elution Kit
Spin Column方式。
Gel Elution回收率可達 60~90%。1.5 ml離心管內萃取洋菜膠核酸樣品可達250 mg，回收後之DNA 可直接進行 enzyme digestion、labeling、PCR、ligation、automated or manual sequencing and T-vector cloning。
操作時間只要15分鐘。

Lane 1: Unpurified DNA fragments
Lane 2, 5, 8: Gel purification by GeneMark Plus Gel Extraction kit
Lane 3, 6, 9: Gel purification by competitor A kit
Lane 4, 7, 10:Gel purification by competitor B kit

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DP04P-Plus PCR Clean-Up Kit
Spin Column方式。
PCR/DNA Clean-Up回收率可達80~95%。
可有效去除primer或dimer。純化後之DNA可直接進行enzyme digestion、labeling、 ligation、hybridization、automated or manual sequencing and T-vector cloning。
操作時間只要15分鐘。

Lane 1: Unpurified PCR product Lane 2: Purified by GeneMark Plus PCR clean-up kit Lane 3: Purified by competitor A kit Lane 4: Purified by competitor B kit

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DP034ME-Micro-Elute DNA Clean/Extraction Kit
相同的buffer, 四種用途.
純化樣本體積大 ─ 純化液態核酸樣品可達 350 μl.
萃取樣本重量重 ─ 萃取洋菜膠核酸樣品可達500 mg.
可將核酸濃度低於ng者濃縮百倍, 不需做酒精沉澱.
回收率>90%, 回收體積最低只要10 μl.
操作時間只要15分鐘.
DNA純化後可直接進行相關分生實驗.

Concentrating DNA and gel purification by Micro-Elute kit
A: DNA Clean-up
Lane 1: 1 ul of unpurified PCR 300 bp product
Lane 2: 10 μl of PCR product was diluted to 1 ml with TE buffer, then processed and eluted to 10 μl using Micro-Elute DNA Clean/Extraction kit. 1 ul of the eluate was loaded onto agarose gel.
Lane 3: Concentrate 500 ul of PCR product to 10 ul volume. Load 1 ul of eluate onto agarose gel.
B: Gel purification
Lane 1: 5 ul of unpurified PCR product
Lane 2: 50 ul eluate of 450 bp DNA fragment was recovered from gel slice by GeneMark DNA Clean/ Extraction kit. Load 5 ul of eluate on agarose gel.
Lane 3: 10 ul eluate of 450 bp DNA fragment was recovered from gel slice by Micro-Elute DNA Clean/Extraction kit. Load 1 ul of eluate on agarose gel.

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