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Modifying Enzymes  分生常用酵素


RNase H ​

E.coli RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double stranded DNA.  

RNase A

Pancreatic, high purity grade. A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA.
​Removal of RNA from preparation of plasmid DNA and preparation of RNA-free DNA.
  

DNase I (RNase-free)

DNase I is lyophilized from Bovine Pancreas, and is essentially RNase-free. It is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing oligodeoxyribonucleotides with 5’-phosphate and 3’-OH groups.
RNase-free DNase I can be used for single- and double-stranded DNA digestion  in RNA purification or any other RNA related applications.
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Figure 1. Gel electrophoresis analysis of total RNA treated with GeneMark RNase-free DNase I (Cat#:GM0649). RNA products were treated with DNase I for 15 min at 37℃, and analyzed on 2 % agarose gel.
Lane 1: 1 μg of total RNA purified using GeneMark total RNA purification kit.
Lane 2: 1 μg of total RNA treated with 2.4 U of DNase I (non-RNase-free).
Lane 3: 1 μg of total RNA treated with 1 U of DNase I (RNase-free).

RNase Inhibitor (DNase RNase-free)

A cDNA encoding human RNase Inhibitor was expressed in E.coli cells, and its recombinant protein was purified to near homogeneity. This product strongly inhibits major nonspecific ribonucleases that include RNase A, B, C and human placental RNase. However, it does not inhibit sequence-specific ribonucleases or ribonuclease activities associated with DNA or RNA polymerases which include RNase T1, S1 nuclease and RNase from Aspergillus sp.

T4 DNA Ligase

A gene encoding bacteriophage T4 DNA Ligase is cloned and expressed in E.coli, and the recombinant T4 DNA Ligase is purified to homogeneity. This enzyme catalyzes the formation of a covalent bond between the 5’-phosphate and 3’-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. Single strands of DNA or RNA can not be ligated with T4 DNA Ligase.

Proteinase K

Proteinase K is a non-specific serine protease isolated from Tritirachium album. It is highly active and stable. The Proteinase K powder is highly soluble (>50 mg/ml), and commonly provided as a 20 mg/ml liquid stock solution. This enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF. 

Picture
Figure 1. BSA digestion using different concentration of Proteinase K.
20 μg BSA is treated with Proteinase K (Prok) of different concentrations (lane 2~4; 1 μg, 0.1 μg. 0.01 μg, respectively). BSA protein can be fully digested when treated with 0.01 μg of Proteinase K.
M: Protein Marker

T7 RNA Polymerase

T7 RNA Polymerase is expressed and purified from E.coli to near homogeneity. This product has increased thermostability. It can utilize the T7 promoter in double-stranded DNA to transcribe a gene located downstream of the promoter.

SP6 RNA Polymerase

SP6 RNA Polymerase catalyzes RNA synthesis in the 5’ to 3’ direction. It requires the presence of a DNA template which contains a SP6 phage promoter. 

T3 RNA Polymerase

T3 RNA Polymerase is highly specific for its own promoter of a conserved 23 bp sequence. This sequence does not efficiently recognize by SP6 or T7 RNA Polymerases. Therefore, without cross-talk from nearby SP6 or T7 promoters, it will transcribe large amounts of RNA from DNA sequences.

Code Description Conc. Size
ZGGD020S Terminal Deoxynucleotidyl Transferase 20U/ul 500U
ZGGD020L Terminal Deoxynucleotidyl Transferase 20U/ul 2500U
ZGGDL039S T4 DNA Ligase, 40U 1U/ul 40U
ZGGDL039M T4 DNA Ligase, 200U 1U/ul 200U
ZGGDL039L T4 DNA Ligase, 1000U 1U/ul 200U*5
ZGGM0649 DNase I (RNase-Free)  2U/ul 2000U
ZGGM0649-10000 DNase I (RNase-Free)  2U/ul 2000U*5
ZGGRB0473-100 RNase A  Pancreatic  High Purity Grade  >60 U/mg 100mg
ZGGRB0473-250 RNase A  Pancreatic  High Purity Grade  >60 U/mg 250mg
ZGGRB0473-500 RNase A  Pancreatic  High Purity Grade >60 U/mg 500mg
ZGGRB0473-1ZGG RNase A  Pancreatic  High Purity Grade >60 U/mg 1g
ZGGD036S RNase H  5 U/μl 250U
ZGGD036L RNase H 5 U/μl 1250U
ZGGRP002AB T7 RNA Polymerase 50U/ul 5000U
ZGGRP003S SP6 RNA Polymerase 20 U/μl 2000U
ZGGRP003L SP6 RNA Polymerase 20 U/μl 10000U
ZGGRP004S T3 RNA Polymerase 20 U/μl 2000U
ZGGRP004L T3 RNA Polymerase 20 U/μl 10000U
B0999 AMV Reverse Transcriptase 10 U/μl 200U
ZGGRT003M SuperSAMscript III Reverse Transcriptase 200 U/μl 10000 U
ZGGRT003L SuperSAMscript III Reverse Transcriptase 200 U/μl 50000 U
ZGGRT004M SuperSAMscript IV Reverse Transcriptase 200 U/μl 10000 U
ZGGRT004L SuperSAMscript IV Reverse Transcriptase 200 U/μl 10000X 5
ZGGRI-M007S GM RNease Inhibitor (DNase RNase free)  40U/ul 2000 U
ZGGRI-M007L GM RNease Inhibitor (DNase RNase free)  40U/ul 2000 UX5
ZGGRI-M007ZGG GM RNease Inhibitor (DNase RNase free)  40U/ul 2000 U X 25
ZGGM-PK0100 Proteinase K  34U/mg 100mg
ZGGM-PK0500 Proteinase K  34U/mg 500 mg
ZGGM-PK1000 Proteinase K  34U/mg 1 g
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力鈞生物科技有限公司
ZGene Biotech Inc.
地址: 104 台北市中山區吉林路245號3樓
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TEL:886-2-25361850 
3F., No.245, Jilin Rd., Zhongshan Dist., Taipei City 104,  Taiwan
Email :
zgenebio.inc@gmail.com ; zgenebio@yahoo.com.tw
line ID:@oev9037t
English web:​https://www.zgenebiotech.com/
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