Modifying Enzymes 分生常用酵素
RNase H
E.coli RNase H (Ribonuclease H) is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA which is hybridized to DNA. This enzyme does not digest single or double stranded DNA.
RNase A
Pancreatic, high purity grade. A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA.
Removal of RNA from preparation of plasmid DNA and preparation of RNA-free DNA.
Removal of RNA from preparation of plasmid DNA and preparation of RNA-free DNA.
DNase I (RNase-free)
DNase I is lyophilized from Bovine Pancreas, and is essentially RNase-free. It is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing oligodeoxyribonucleotides with 5’-phosphate and 3’-OH groups.
RNase-free DNase I can be used for single- and double-stranded DNA digestion in RNA purification or any other RNA related applications.
RNase-free DNase I can be used for single- and double-stranded DNA digestion in RNA purification or any other RNA related applications.
|
Figure 1. Gel electrophoresis analysis of total RNA treated with GeneMark RNase-free DNase I (Cat#:GM0649). RNA products were treated with DNase I for 15 min at 37℃, and analyzed on 2 % agarose gel.
Lane 1: 1 μg of total RNA purified using GeneMark total RNA purification kit. Lane 2: 1 μg of total RNA treated with 2.4 U of DNase I (non-RNase-free). Lane 3: 1 μg of total RNA treated with 1 U of DNase I (RNase-free). |
RNase Inhibitor (DNase RNase-free)
A cDNA encoding human RNase Inhibitor was expressed in E.coli cells, and its recombinant protein was purified to near homogeneity. This product strongly inhibits major nonspecific ribonucleases that include RNase A, B, C and human placental RNase. However, it does not inhibit sequence-specific ribonucleases or ribonuclease activities associated with DNA or RNA polymerases which include RNase T1, S1 nuclease and RNase from Aspergillus sp.
T4 DNA Ligase
A gene encoding bacteriophage T4 DNA Ligase is cloned and expressed in E.coli, and the recombinant T4 DNA Ligase is purified to homogeneity. This enzyme catalyzes the formation of a covalent bond between the 5’-phosphate and 3’-OH in nicked duplex DNA or at two DNA ends. This activity is very useful to ligate DNA fragments with either cohesive or blunt ends that are generated by restriction enzyme digestion. T4 DNA Ligase can also ligate RNA with DNA or RNA in a double helix with low efficiency. Single strands of DNA or RNA can not be ligated with T4 DNA Ligase.
Proteinase K
Proteinase K is a non-specific serine protease isolated from Tritirachium album. It is highly active and stable. The Proteinase K powder is highly soluble (>50 mg/ml), and commonly provided as a 20 mg/ml liquid stock solution. This enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
|
Figure 1. BSA digestion using different concentration of Proteinase K.
20 μg BSA is treated with Proteinase K (Prok) of different concentrations (lane 2~4; 1 μg, 0.1 μg. 0.01 μg, respectively). BSA protein can be fully digested when treated with 0.01 μg of Proteinase K. M: Protein Marker |
T7 RNA Polymerase
T7 RNA Polymerase is expressed and purified from E.coli to near homogeneity. This product has increased thermostability. It can utilize the T7 promoter in double-stranded DNA to transcribe a gene located downstream of the promoter.
SP6 RNA Polymerase
SP6 RNA Polymerase catalyzes RNA synthesis in the 5’ to 3’ direction. It requires the presence of a DNA template which contains a SP6 phage promoter.
T3 RNA Polymerase
T3 RNA Polymerase is highly specific for its own promoter of a conserved 23 bp sequence. This sequence does not efficiently recognize by SP6 or T7 RNA Polymerases. Therefore, without cross-talk from nearby SP6 or T7 promoters, it will transcribe large amounts of RNA from DNA sequences.
| Code | Description | Conc. | Size |
| ZGGD020S | Terminal Deoxynucleotidyl Transferase | 20U/ul | 500U |
| ZGGD020L | Terminal Deoxynucleotidyl Transferase | 20U/ul | 2500U |
| ZGGDL039S | T4 DNA Ligase, 40U | 1U/ul | 40U |
| ZGGDL039M | T4 DNA Ligase, 200U | 1U/ul | 200U |
| ZGGDL039L | T4 DNA Ligase, 1000U | 1U/ul | 200U*5 |
| ZGGM0649 | DNase I (RNase-Free) | 2U/ul | 2000U |
| ZGGM0649-10000 | DNase I (RNase-Free) | 2U/ul | 2000U*5 |
| ZGGRB0473-100 | RNase A Pancreatic High Purity Grade | >60 U/mg | 100mg |
| ZGGRB0473-250 | RNase A Pancreatic High Purity Grade | >60 U/mg | 250mg |
| ZGGRB0473-500 | RNase A Pancreatic High Purity Grade | >60 U/mg | 500mg |
| ZGGRB0473-1ZGG | RNase A Pancreatic High Purity Grade | >60 U/mg | 1g |
| ZGGD036S | RNase H | 5 U/μl | 250U |
| ZGGD036L | RNase H | 5 U/μl | 1250U |
| ZGGRP002AB | T7 RNA Polymerase | 50U/ul | 5000U |
| ZGGRP003S | SP6 RNA Polymerase | 20 U/μl | 2000U |
| ZGGRP003L | SP6 RNA Polymerase | 20 U/μl | 10000U |
| ZGGRP004S | T3 RNA Polymerase | 20 U/μl | 2000U |
| ZGGRP004L | T3 RNA Polymerase | 20 U/μl | 10000U |
| B0999 | AMV Reverse Transcriptase | 10 U/μl | 200U |
| ZGGRT003M | SuperSAMscript III Reverse Transcriptase | 200 U/μl | 10000 U |
| ZGGRT003L | SuperSAMscript III Reverse Transcriptase | 200 U/μl | 50000 U |
| ZGGRT004M | SuperSAMscript IV Reverse Transcriptase | 200 U/μl | 10000 U |
| ZGGRT004L | SuperSAMscript IV Reverse Transcriptase | 200 U/μl | 10000X 5 |
| ZGGRI-M007S | GM RNease Inhibitor (DNase RNase free) | 40U/ul | 2000 U |
| ZGGRI-M007L | GM RNease Inhibitor (DNase RNase free) | 40U/ul | 2000 UX5 |
| ZGGRI-M007ZGG | GM RNease Inhibitor (DNase RNase free) | 40U/ul | 2000 U X 25 |
| ZGGM-PK0100 | Proteinase K | 34U/mg | 100mg |
| ZGGM-PK0500 | Proteinase K | 34U/mg | 500 mg |
| ZGGM-PK1000 | Proteinase K | 34U/mg | 1 g |
