TriSolution Plus Reagent (RNAzol / RNA isolater Reagent)
ZGGMTS100-PLUS 100ml
ZGGMTS200-PLUS 200ml
Application：
Suitable for isolating RNA, DNA, and protein from a variety of species including animal, plant, and cell.
Product introduction：
TriSolution Plus Reagent is a mixture of phenol, guanidium thiocynate, buffers and stabilizers developed for the simultaneously isolation of total RNA, DNA and proteins from animal and plant tissues, cells and bacteria culture. The entire procedure for total RNA isolation is an improved single-step method developed by Chomczynski and Sacchi. After homogenization of sample and chloroform extraction, three phases are formed (aqueous phase, interphase and organic phase). RNA can be precipitated by isopropanol from aqueous phase, DNA can be recovered by ethanol precipitation from interphase, and proteins are precipitated with isopropanol from organic phase. The reagent also includes a bottle of PS & PG Removal Solution, which can eliminate polysaccharides and proteoglycans contamination, by which the RNA can be dissolved easier and purer for RT-PCR and northern blot application.
ZGGMTS200-PLUS 200ml
Application：
Suitable for isolating RNA, DNA, and protein from a variety of species including animal, plant, and cell.
Product introduction：
TriSolution Plus Reagent is a mixture of phenol, guanidium thiocynate, buffers and stabilizers developed for the simultaneously isolation of total RNA, DNA and proteins from animal and plant tissues, cells and bacteria culture. The entire procedure for total RNA isolation is an improved single-step method developed by Chomczynski and Sacchi. After homogenization of sample and chloroform extraction, three phases are formed (aqueous phase, interphase and organic phase). RNA can be precipitated by isopropanol from aqueous phase, DNA can be recovered by ethanol precipitation from interphase, and proteins are precipitated with isopropanol from organic phase. The reagent also includes a bottle of PS & PG Removal Solution, which can eliminate polysaccharides and proteoglycans contamination, by which the RNA can be dissolved easier and purer for RT-PCR and northern blot application.
|
圖片說明：右為 I 牌公司TRIzol 純化結果，左為ZGENE ZTRIpure
The picture shows the RNA quality of extraction from mouse liver with two different reagent: TRIzol (Ixxxxxxgen) on the right , and ZTRIpure on the left. |
- RNA Keeper Tissue Stabilizer RNA 組織樣本保存液
|
訂購資訊 : ZGVR501-01 100ml
方便的用於保護RNA完整性的試劑
• 樣本無需液氮速凍 • 立即穩定並保護RNA • 可靠的基因表達譜資料 • 可長期保存組織樣本 |
圖1. 將小鼠肝臟（1）和脾臟（2）保存在RNA Keeper中，分別在37℃放置1天，室溫放置1周，4℃放置4周後，用RNA Isolater 提取總RNA。電泳結果表明，28 s、18 s、5 s條帶均清晰可見，提示RNA具有很好的完整性。
|
產品描述
RNA Keeper Tissue Stabilizer是一種無毒的溶液，可迅速滲入組織，滅活內源RNase，立即穩定並保護RNA的完整性。新鮮的組織樣本無需液氮速凍，只要浸入RNA Keeper Tissue Stabilizer中，即可在37℃保存1天，室溫保存1周，4℃保存4周或在-20℃/-80℃長期保存，且反復凍融不會顯著影響RNA的完整性。RNA Keeper Tissue Stabilizer中存放的樣本可直接使用RNA isolater總RNA提取試劑或離心柱法提取RNA。RNA Keeper Tissue Stabilizer可用於腦、心臟、肝臟、胰臟、腎臟、脾臟、睾丸、肌肉等組織的保存。
RNA Keeper Tissue Stabilizer是一種無毒的溶液，可迅速滲入組織，滅活內源RNase，立即穩定並保護RNA的完整性。新鮮的組織樣本無需液氮速凍，只要浸入RNA Keeper Tissue Stabilizer中，即可在37℃保存1天，室溫保存1周，4℃保存4周或在-20℃/-80℃長期保存，且反復凍融不會顯著影響RNA的完整性。RNA Keeper Tissue Stabilizer中存放的樣本可直接使用RNA isolater總RNA提取試劑或離心柱法提取RNA。RNA Keeper Tissue Stabilizer可用於腦、心臟、肝臟、胰臟、腎臟、脾臟、睾丸、肌肉等組織的保存。
