ZTRIpure Total RNA Reagent
ZGRN0101 50ml /ZGRN0102 100ml /ZGRN0103 200ml
Application：
Suitable for isolating RNA, DNA, and protein from a variety of species including animal, plant, and cell.
Product introduction：
ZTRIpure reagent is used to directly purify RNA. It could keep the integrity of RNA in damaged cells. After adding chloroform and centrifuge, sample could be separated into aqueous and organic phase. RNA involves in the aqueous phase. Add the isopropanol to the aqueous phase, and RNA precipitate presents. After removing the aqueous phase, DNA and the protein in the sample could also separate out. Adding the ethanol to the interphase could get DNA precipitate, and the isopropanol to the organic phase could get the protein precipitate. DNA purification is essential to standardize the RNA yield.
The kit performs good separation capability on whether human, animal, plant, or bacteria and either for small number of or large number of tissues and cells. The operation of ZTRIpure reagent is so much easy so that many samples could be conducted at the same time. The whole procedures only take for an hour. It could prevent the contamination of DNA and the protein. Therefore, it could be used in RNA northern blotting, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, or molecular cloning. If used in PCR, it is suggested to purify RNA with the amplification grade DNase I when two primers locate at a single exon.
ZTRIpure reagent could facilitate the RNA precipitate with different species and different molecular weight. For an example, RNA from the liver of the rat runs on the agarose gel, stained with EtBr, and it presents discontinuous bands with high molecular weight from 7kb to 15 kb(mRNA and hnRNA). The two main ribosomal RNA are ~5 kb (28S) and ~2 kb(18S), and RNA with low molecular weight is ranged from 0.1 to 0.3 kb(tRNA, 5S). The ratio A260/A280 is more than or equal to 1.8 with the isolated RNA diluted with TE buffer.
Application：
Suitable for isolating RNA, DNA, and protein from a variety of species including animal, plant, and cell.
Product introduction：
ZTRIpure reagent is used to directly purify RNA. It could keep the integrity of RNA in damaged cells. After adding chloroform and centrifuge, sample could be separated into aqueous and organic phase. RNA involves in the aqueous phase. Add the isopropanol to the aqueous phase, and RNA precipitate presents. After removing the aqueous phase, DNA and the protein in the sample could also separate out. Adding the ethanol to the interphase could get DNA precipitate, and the isopropanol to the organic phase could get the protein precipitate. DNA purification is essential to standardize the RNA yield.
The kit performs good separation capability on whether human, animal, plant, or bacteria and either for small number of or large number of tissues and cells. The operation of ZTRIpure reagent is so much easy so that many samples could be conducted at the same time. The whole procedures only take for an hour. It could prevent the contamination of DNA and the protein. Therefore, it could be used in RNA northern blotting, dot blot hybridization, poly(A)+ selection, in vitro translation, RNase protection assay, or molecular cloning. If used in PCR, it is suggested to purify RNA with the amplification grade DNase I when two primers locate at a single exon.
ZTRIpure reagent could facilitate the RNA precipitate with different species and different molecular weight. For an example, RNA from the liver of the rat runs on the agarose gel, stained with EtBr, and it presents discontinuous bands with high molecular weight from 7kb to 15 kb(mRNA and hnRNA). The two main ribosomal RNA are ~5 kb (28S) and ~2 kb(18S), and RNA with low molecular weight is ranged from 0.1 to 0.3 kb(tRNA, 5S). The ratio A260/A280 is more than or equal to 1.8 with the isolated RNA diluted with TE buffer.
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圖片說明：右為 I 牌公司TRIzol 純化結果，左為ZGENE ZTRIpure
The picture shows the RNA quality of extraction from mouse liver with two different reagent: TRIzol (Ixxxxxxgen) on the right , and ZTRIpure on the left. |
EASYspin
Plant RNA Kit
EASYspin 植物RNA快速純化試劑盒
EASYspin 植物RNA快速純化試劑盒
EASY microRNA Mini Kit
植物小RNA快速純化試劑盒
植物小RNA快速純化試劑盒
Description
The EASY Mini Kit combines phenol/guanidine-based lysis of samples and silicamembrane based purification of total RNA. Lysis/Binding buffer is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
Cells or tissue samples are homogenized in Lysis/Binding buffer. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase, while DNA partitions to the interphase and proteins to the lower, organic phase or the interphase.
The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions for all RNA molecules from 18 nucleotides (nt) upwards. The sample is then applied to the RNA Bind columns, where the total RNA binds to the membrane and phenol and other contaminants are efficiently washed away. Highquality RNA is then eluted in RNase-free water.
For enrichment of miRNAs and other small RNAs (less than ~200 nt) in a separate fraction, a specialized protocol is provided in Appendix A, page 31. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. For this specialized protocol, an additional microRNA Bind Columns is inclued.
v Features
1. Effective purification of miRNA and total RNA
2. Efficient enrichment of miRNA and RNAs <200 nucleotides
3. High-purity RNA suitable for all downstream applications
4. Protocols for copurification or isolation of separate fractions
The EASY Mini Kit combines phenol/guanidine-based lysis of samples and silicamembrane based purification of total RNA. Lysis/Binding buffer is a monophasic solution of phenol and guanidine thiocyanate, designed to facilitate lysis of tissues, to inhibit RNases, and also to remove most of the cellular DNA and proteins from the lysate by organic extraction.
Cells or tissue samples are homogenized in Lysis/Binding buffer. After addition of chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase, while DNA partitions to the interphase and proteins to the lower, organic phase or the interphase.
The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions for all RNA molecules from 18 nucleotides (nt) upwards. The sample is then applied to the RNA Bind columns, where the total RNA binds to the membrane and phenol and other contaminants are efficiently washed away. Highquality RNA is then eluted in RNase-free water.
For enrichment of miRNAs and other small RNAs (less than ~200 nt) in a separate fraction, a specialized protocol is provided in Appendix A, page 31. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. For this specialized protocol, an additional microRNA Bind Columns is inclued.
v Features
1. Effective purification of miRNA and total RNA
2. Efficient enrichment of miRNA and RNAs <200 nucleotides
3. High-purity RNA suitable for all downstream applications
4. Protocols for copurification or isolation of separate fractions
- RNA Keeper Tissue Stabilizer RNA 組織樣本保存液
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訂購資訊 : ZGVR501-01 100ml
方便的用於保護RNA完整性的試劑
• 樣本無需液氮速凍 • 立即穩定並保護RNA • 可靠的基因表達譜資料 • 可長期保存組織樣本 |
圖1. 將小鼠肝臟（1）和脾臟（2）保存在RNA Keeper中，分別在37℃放置1天，室溫放置1周，4℃放置4周後，用RNA Isolater 提取總RNA。電泳結果表明，28 s、18 s、5 s條帶均清晰可見，提示RNA具有很好的完整性。
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產品描述
RNA Keeper Tissue Stabilizer是一種無毒的溶液，可迅速滲入組織，滅活內源RNase，立即穩定並保護RNA的完整性。新鮮的組織樣本無需液氮速凍，只要浸入RNA Keeper Tissue Stabilizer中，即可在37℃保存1天，室溫保存1周，4℃保存4周或在-20℃/-80℃長期保存，且反復凍融不會顯著影響RNA的完整性。RNA Keeper Tissue Stabilizer中存放的樣本可直接使用RNA isolater總RNA提取試劑或離心柱法提取RNA。RNA Keeper Tissue Stabilizer可用於腦、心臟、肝臟、胰臟、腎臟、脾臟、睾丸、肌肉等組織的保存。
RNA Keeper Tissue Stabilizer是一種無毒的溶液，可迅速滲入組織，滅活內源RNase，立即穩定並保護RNA的完整性。新鮮的組織樣本無需液氮速凍，只要浸入RNA Keeper Tissue Stabilizer中，即可在37℃保存1天，室溫保存1周，4℃保存4周或在-20℃/-80℃長期保存，且反復凍融不會顯著影響RNA的完整性。RNA Keeper Tissue Stabilizer中存放的樣本可直接使用RNA isolater總RNA提取試劑或離心柱法提取RNA。RNA Keeper Tissue Stabilizer可用於腦、心臟、肝臟、胰臟、腎臟、脾臟、睾丸、肌肉等組織的保存。
