Genemark RNA Isolation Kit   RNA 純化試劑組

Total RNA Miniprep Purification Kit (TR01)

•適用樣品: 細菌(109)、動物組織(30 mg)、 培養細胞(107)、血液(0.5 ml fresh blood)
•純化方式: silica membrane spin column
•RNA產量: 10~100μg /reaction
•無需phenol/chloroform及酒精沉澱
•附有DNase I幫助去除genomic DNA的干擾

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Non-denaturing gel electrophoresis of the total RNA purified from various samples by GeneMark and Q supplier. The RNA from Q supplier contains large amount of genomic DNA contamination.

Plant Total RNA Miniprep Purification Kit (TR02)

•適用樣品: 各種植物(100 mg)、真菌
•純化方式: silica membrane spin column
•RNA產量: 10~100μg /reaction
•無需phenol/chloroform及酒精沉澱
•特殊配方，有效去除植物組織內高量的 polyphenolics及polysaccharides
•附有DNase I幫助去除genomic DNA的干擾

不必再害怕處理多醣體較多及高黏度的植物組織了!!
​針對不同植物組織，提供2種萃取液供您選擇!​

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Lysis Solution A
Lysis Solution B
Major component
guanidinium thiocyanate
SDS/anti-oxidant
Application
Most plant tissues,
BUT NOT for high starch, polyphenol polysaccharides samples.
Broadest range of plant tissues including high starch, polyphenolics, polysaccharides samples,BUT NOT for orchids and pineapple leaves.
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​Figure 1. Non-denaturing gel electrophoresis analysis of the total RNA purified from various plant leaf samples using GeneMark Plant Total RNA Purification Kit with Lysis Solution B system, and related kit from supplier Q. The rice RNA purified using related kit from supplier Q contains large amount of genomic DNA contamination.
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Figure 2. Gel electrophoresis analysis of RT-PCR of rice actin gene. Total RNA was purified using GeneMark Plant Total RNA Purification Kit and related kit from supplier Q. Genomic DNA contamination was observed from RNA purified using kit from supplier Q in both RT and Taq only control.