Plant chloroplast genome NGS sequencing and assembling
Chloroplast DNA is an organelle genomes that exist independently outside the nucleus genome, with separate transcription and translation system. The size of chloroplast genome is far less than nucleus genome, approximately one ten thousandth of its size. The cell of chloroplast genome has the copy number ranging from 1000 to 10,000. The average size of the genome is between 120-180 kb, double-strand structure, mainly maternally inherited. The main structure of chloroplast genome consists of large single-copy (LSC), small single-copy (SSC) region and two inverted repeats region (IRA & IRB). Most chloroplast genome is highly conservative, and are widely used in plant DNA barcoding and the analysis of the species evolution. The specific segments used for DNA barcoding are rbcL (1,400, bp), trnL-trnF (250-800 bp), atpB-rbcL (1,000 bp), trnL intron (300 bp), matK (2,600 bp), trnT-trnL (400-800 bp), 16S (1,400 bp) and rpoC (3,600 bp) and so on.Until September 2014, there had been 1204 species of complete chloroplast genome were published on NCBI database. By contrast, it is not as many as 38,778 species’ mitochondria genome publication. The main reason is that chloroplast genome of plants is ten times larger than mitogenome of animals. The traditional way of DNA sequencing using Sanger sequencing or BAC / fosmid sequencing method are relatively inefficient, which results in the slow progress of sequencing in the complete chloroplast genome. Wethink Biotech INC. adopts the latest NGS sequencing method using Illumina sequencing instrument with optimized de novo assembly program which can effectively parse out the intact chloroplast genome of algae, gymnosperms and angiosperms (70-200 kb), and leads to a great progress on plant evolution and utilization of chloroplast genetic engineering
Chloroplast genome Mitochondrial genome sequencing services
(1)Containing a standard establishing DNA library and NGS sequencing
(2)Containing a de novo and assemble into a circular form DNA
(3)Containing standard sequence analysis and annotation
(4)Containing precise circular form DNA map diagram output
(5)Containing free consulting services of paper published
(2)Containing a de novo and assemble into a circular form DNA
(3)Containing standard sequence analysis and annotation
(4)Containing precise circular form DNA map diagram output
(5)Containing free consulting services of paper published
Chloroplast genome sequencing service quality requirement
Sample requirement (complete chloroplast DNA or genomic DNA or fresh plant tissue with chloroplast)
(1)Purity: OD 260/280 value between 1.8~2.0
(2)Concentration: above 100 ng/µl for each sample
(3)Total Volume: above 2 µg for each sample
(4)Solution: ddH2O or TE buffer (pH 8.0)
(1)Purity: OD 260/280 value between 1.8~2.0
(2)Concentration: above 100 ng/µl for each sample
(3)Total Volume: above 2 µg for each sample
(4)Solution: ddH2O or TE buffer (pH 8.0)