ZGeneBio Fetal DNA in Maternal Plasma Extraction Kit
Cat# ZGCIR02-20/50
Size : 20, 50 Reactions
For concentration and purification of Fetal DNA from Maternal plasma, serum or Urine
NGS / QPCR / PCR
Introduction
Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and
maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis. The detection of fetal DNA in maternal plasma is much simpler and more robust than detecting fetal nucleated cells in maternal blood, and does not require prior enrichment. During pregnancy, the fetal and maternal circulations are separated by the placental membranes. However, a variety of evidence has
pointed toward the incompleteness of this barrier to cellular trafficking. Of special relevance to this review, fetal nucleated cells have been demonstrated in maternal circulation and have been widely pursued as potential substrates for noninvasive prenatal diagnosis Based on the high efficiency and specificity between nucleic acid and ZGeneBio beads. ZGeneBio developed the Fetal DNA Extraction Kit from Maternal plasma.
The maternal plasma and serum have been shown to contain fetal DNA. For plasma DNA detection, several recent studies addressed
the need for careful evaluation and standardization of preanalytical processes. Key problems appear, such as possible contamination of plasma by white blood cells; the generally low and variable amount of free circulating DNA, making extraction/quantification difficult and time-consuming; poor DNA quality; and the presence of PCR inhibitors. In any case, automation of DNA extraction, which is a prerequisite for introduction of these diagnostic approaches in clinical laboratories, is difficult to achieve because of the volumes of plasma necessary to get sufficient DNA.
The ZGeneBio Fetal DNA in Maternal Plasma Extraction Kit is a simple, rapid method and time-saving process, which can be proutinely used in laboratories. The entire procedure takes less than 1 hour as many as 12 samples can be handled in one cycle. It also enables efficient
purification of these free circulating nucleic acids and provides high yields from human plasma or serum. The kit could starting sample volumes of up to 10 ml, and flexible elution volumes between 50 μl and 150 μl allow concentration of nucleic acid species that are present in low amounts in the sample material. The kit can also be used for purification and concentration of viral nucleic acids from large sample volumes.
Cat# ZGCIR02-20/50
Size : 20, 50 Reactions
For concentration and purification of Fetal DNA from Maternal plasma, serum or Urine
NGS / QPCR / PCR
Introduction
Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and
maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis. The detection of fetal DNA in maternal plasma is much simpler and more robust than detecting fetal nucleated cells in maternal blood, and does not require prior enrichment. During pregnancy, the fetal and maternal circulations are separated by the placental membranes. However, a variety of evidence has
pointed toward the incompleteness of this barrier to cellular trafficking. Of special relevance to this review, fetal nucleated cells have been demonstrated in maternal circulation and have been widely pursued as potential substrates for noninvasive prenatal diagnosis Based on the high efficiency and specificity between nucleic acid and ZGeneBio beads. ZGeneBio developed the Fetal DNA Extraction Kit from Maternal plasma.
The maternal plasma and serum have been shown to contain fetal DNA. For plasma DNA detection, several recent studies addressed
the need for careful evaluation and standardization of preanalytical processes. Key problems appear, such as possible contamination of plasma by white blood cells; the generally low and variable amount of free circulating DNA, making extraction/quantification difficult and time-consuming; poor DNA quality; and the presence of PCR inhibitors. In any case, automation of DNA extraction, which is a prerequisite for introduction of these diagnostic approaches in clinical laboratories, is difficult to achieve because of the volumes of plasma necessary to get sufficient DNA.
The ZGeneBio Fetal DNA in Maternal Plasma Extraction Kit is a simple, rapid method and time-saving process, which can be proutinely used in laboratories. The entire procedure takes less than 1 hour as many as 12 samples can be handled in one cycle. It also enables efficient
purification of these free circulating nucleic acids and provides high yields from human plasma or serum. The kit could starting sample volumes of up to 10 ml, and flexible elution volumes between 50 μl and 150 μl allow concentration of nucleic acid species that are present in low amounts in the sample material. The kit can also be used for purification and concentration of viral nucleic acids from large sample volumes.