ZGeneBio Low cell density genomic DNA extraction Kit are designed specific to purify of total DNA from low cell density (from 10^2 to10^4) using a safe protocol that eliminates the use of organic solvents such as phenol and chloroform .By using proteinase K and a lysis buffer to effectively lyses the cells and denature the protein. The DNA in the chaotropic salt are high efficiency and specificity bound to ZGeneBio beads. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA are eluted by a low salt based elution buffer or water. The entire procedure can be completed in 30 minutes, and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reactions.
1. Simple，Convenient，Rapid: The protocol only take 30 min
2. Reduce the cell culture time: 1-2ug DNA from single cell culture102-104
3. High quality DNA: The DNA is ready for q-PCR or others assay
4. Low cost: Reduce the cost for cell culture medium and material
5. Fast: Save the time for cell culture. . It is the best tool for cell mutation selection