T4 DNA Ligase
ZGFL101-01 10000 units
該酶從表達T4基因的大腸桿菌中純化而來。它催化雙鏈DNA或RNA的5'-磷酸末端和3' - 羥基形成磷酸二酯鍵。該酶催化平滑末端或粘性末端DNA的連接，修復雙鏈DNA，RNA，DNA/RNA雜交鏈中的單鏈切口
反應條件:
TA Cloning :25度10分鐘
平端黏合:25度2小時或16度過夜
快速TA Cloning kit 5分鐘快速黏合
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pEASY®-T1 Cloning Kit
C101-01 20RXN Description pEASY®-T1 Cloning Kit is designed for cloning and sequencing Taq amplified PCR products. • 5 minutes fast ligation of Taq-amplified PCR products. • Kanamycin and Ampicillin resistance genes for your choice of selection. • Easy blue/white selection. • T7 promoter primer, M13 forward primer and M13 reverse primer for sequencing. • T7 promoter for in vitro transcription. • Trans1-T1 phage resistant chemically competent cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Features： 1. 5 minutes fast ligation of Taq-amplified PCR products. 2.Kanamycin and Ampicillin resistance genes for your choice of selection. 3.Easy blue/white selection. 4. T7 promoter primer, M13 forward primer and M13 reverse primer for sequencing. 5. T7 promoter |
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pEASY-T3 Cloning Kit
CT301-01 20RXN Description pEASY ®- T3 Cloning Vector provides dual-EcoR I and dual-Not I enzyme digestion sites. It is designed for cloning and sequencing Taq amplified PCR products. The cloned insert can be released from a single enzyme digestion. • 5 minutes fast ligation of Taq-amplified PCR products. • Ampicillin resistance genes for your choice of selection. • Easy blue/white selection. • T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. • T7 promoter for in vitro transcription. • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Features： 1. 5 minutes fast ligation of Taq-amplified PCR products. 2. Ampicillin resistance genes for your choice of selection. 3. Easy blue/white selection. 4. T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. 5. T7 promoter for in vitro |
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pEASY ®-T5 Zero Cloning KIT 零背景 不用藍白篩
Description pEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required. • 5 minutes fast ligation of Taq-amplified PCR products. • High cloning efficiency. Positive clones up to 100%. • No blue/white selection needed. • Suitable for short and large fragment cloning. • Kanamycin and Ampicillin resistance genes for choice of selection. • M13 forward primer and M13 reverse primer for sequencing. • T3 promoter and T7 promoter for in vitro transcription. • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Features： 1. 5 minutes fast ligation of Taq-amplified PCR products. 2.High cloning efficiency. Positive clones up to 100%. 3.No blue/white selection needed. 4.Suitable for short and large fragment cloning. 5.Kanamycin and Ampicillin resistance genes for choice of selec |
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pEASY Blunt Cloning Kit
ZGCB101-01 20RXN Description pEASY Blunt Cloning Kit is designed for cloning and sequencing Pfu-amplified PCR products. • 5 minutes fast ligation of Pfu-amplified PCR products. • Kanamycin and Ampicillin resistance genes for your choice of selection. • Easy blue/white selection. • T7 promoter, M13 forward and M13 reverse primers for sequencing. • T7 promoter for in vitro transcription. • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing. Features： 1.5 minutes fast ligation of Pfu-amplified PCR products. 2.Kanamycin and Ampicillin resistance genes for your choice of selection. 3.Easy blue/white selection. 4.T7 promoter, M13 forward and M13 reverse primers for sequencing. 5.T7 promoter for in vitro trans |
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DNA Ladder
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Taq ,高保真酵素
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引子合成服務
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RT 反轉錄
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游離DNA KIT
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Viogene Plasmid KIT
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