快速 T3 TA Cloning kit 5分鐘快速黏合 高成功率 室溫5min快速Cloning 操作簡單:只需要加入載體及PCR Product 效率高:比傳統T4 Ligase 成功率高 成功率高達90%以上雙抗生素篩選
pEASY-T3 Cloning Kit CT301-01 20RXN Description pEASY ®- T3 Cloning Vector provides dual-EcoR I and dual-Not I enzyme digestion sites. It is designed for cloning and sequencing Taq amplified PCR products. The cloned insert can be released from a single enzyme digestion. • 5 minutes fast ligation of Taq-amplified PCR products. • Ampicillin resistance genes for your choice of selection. • Easy blue/white selection. • T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. • T7 promoter for in vitro transcription. • Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Features: 1. 5 minutes fast ligation of Taq-amplified PCR products. 2. Ampicillin resistance genes for your choice of selection. 3. Easy blue/white selection. 4. T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing. 5. T7 promoter for in vitro