ZGeneBio Small Fragment PCR Product Gel Extraction / Clean Up Kit
Cat# ZGCIR05-20/50
Size : 20/ 50 Reactions
Specific for small fragment PCR product gel extraction Purification of double- or single-stranded PCR products Cleanup of oligonucleotides and DNA from enzymatic reactions
Introduction
ZGeneBio Small Fragment PCR Product Gel Extraction Kit are designed specific to recover or concentrate small DNA fragments (50bp-300bp) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and
denature the enzymes. The DNA fragments in the chaotropic salt are high efficiency and specificity bound to ZGeneBio beads. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are 80-90% for gel extraction and 90-95% for PCR clean-up. The entire procedure can be completed in 30 minutes , and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reactions.
Cat# ZGCIR05-20/50
Size : 20/ 50 Reactions
Specific for small fragment PCR product gel extraction Purification of double- or single-stranded PCR products Cleanup of oligonucleotides and DNA from enzymatic reactions
Introduction
ZGeneBio Small Fragment PCR Product Gel Extraction Kit are designed specific to recover or concentrate small DNA fragments (50bp-300bp) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and
denature the enzymes. The DNA fragments in the chaotropic salt are high efficiency and specificity bound to ZGeneBio beads. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are 80-90% for gel extraction and 90-95% for PCR clean-up. The entire procedure can be completed in 30 minutes , and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reactions.
