突變篩選工具
T7 核酸內切酶 I ( T7E1 突變篩選酶試劑組 )
T7 endonuclease I T7E1 T7EI 應用
■ 基因突變和SNP的檢測，可應用於TALEN、CRISPR/CAS9形成的突變體檢測 ■ 突變篩選識別錯配(mismatch) DNA，分解四方向交叉 DNA 或分支 DNA ■ 檢測或切割異源二聚體 DNA 和切割 DNA ■ 隨機切割線性 DNA 進行 shot-gun 克隆 |
細胞培養小幫手
黴漿菌快速檢試劑組
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T7E1酶切法檢測突變體實驗步驟
1、 PCR放大出帶有突變位點 (如TALEN or CRISPR/Cas9的target site) DNA片段，長度約500bp，突變位點最好不位於PCR片段中央，這樣將切割
出兩條大小不同的帶。
2、 突變體DNA與野生型DNA的PCR產物按進行混合，進行加熱denature、annealing複性處理。
3、上述反應分別加入0.5ul T7E1酶，37℃反應30 min後，立刻跑2%的Agarose電泳檢測分析酶切結果。（放置時間過長可能會導致PCR產物
全部降解。
1、 PCR放大出帶有突變位點 (如TALEN or CRISPR/Cas9的target site) DNA片段，長度約500bp，突變位點最好不位於PCR片段中央，這樣將切割
出兩條大小不同的帶。
2、 突變體DNA與野生型DNA的PCR產物按進行混合，進行加熱denature、annealing複性處理。
3、上述反應分別加入0.5ul T7E1酶，37℃反應30 min後，立刻跑2%的Agarose電泳檢測分析酶切結果。（放置時間過長可能會導致PCR產物
全部降解。
T7 Endonuclease I T7E1 Digestion
T7E1 可針對DNA Mismatch 進行切割 來進行突變篩選
T7E1 可針對DNA Mismatch 進行切割 來進行突變篩選
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Order info
T7 endonuclease I T7E1 500U ZG-T7E1001 500U T7 endonuclease I T7E1 1000U ZG-T7E1002 1000U T7 endonuclease I T7E1 1500U ZGBT7E1002 1500U (0.5U/ul) |
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HRM 高熔點分析
ZGB EVA Green qPCR Mix
ZGB EVA Green qPCR Mix
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Cas9 DNA nuclease Kit Cas9 體外酶切gRNA 切割效率檢測酶
Cas9 體外酶切gRNA
切割效率檢測酶
編號 :ZGVN301-01(50 pmol)
編號 :ZGVN301-01(50 pmol)
Cas9 Nuclease是一個RNA導向的序列專一性雙鏈DNA內切酶。嚮導RNA通過與靶序列互補來識別靶位點，並將Cas9 Nuclease帶到靶 DNA上。Cas9 Nuclease有兩個切割活性中心，分別切割靶DNA的兩條鏈從而產生DNA雙鏈斷裂。切割位點為靶向區域內和NGGPAM 相距三個鹼基處。本產品是克隆Streptococcus pyogenes的Cas9基因後在大腸桿菌中表達純化的高純度Cas9活性蛋白
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dsDNA (500bp)
200 ng
gRNA (體外轉錄) 100 ng Cas9 1 ul 10X Buffer 3 ul ddH2O x ul Total 20 ul 充分混合後，37℃反應1h，65℃煮5 min, 跑膠檢測分析檢測酵素反應結果 |
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Anti-cas9 antibody
Product name： Anti-Cas9抗體
Description : Rabbit polyclonal antibody to Cas9
Tested applications： WB, ICC/IF
Species reactivity Reacts with: Streptococcus pyogenes Cas9
Immunogen： Recombinant full length S.pyogenes Cas9
Positive control WB: E.coli expression of Recombinant Cas9; IF: HEK-293 cells expressing Cas9
Description : Rabbit polyclonal antibody to Cas9
Tested applications： WB, ICC/IF
Species reactivity Reacts with: Streptococcus pyogenes Cas9
Immunogen： Recombinant full length S.pyogenes Cas9
Positive control WB: E.coli expression of Recombinant Cas9; IF: HEK-293 cells expressing Cas9
NHEJ Inhibitor SCR7
5,6-bis((E)-benzylideneamino)-2-mercaptopyrimidin-4-ol
SCR7 is a potent and selective inhibitor of non-homologous end joining (NHEJ). It inhibits joining of DSBs in cell-free DNA repair system, blocks Ligase IV-mediated joining by interfering with its DNA binding but not that of T4 DNA Ligase or Ligase I, thereby leading to accumulation of DSBs within the cells, culminating into cytotoxicity. SCR7 inhibits NHEJ in a Ligase IV-dependent manner within cells, and activates the intrinsic apoptotic pathway. More importantly, SCR7 impedes tumor progression in mouse models, and when co-administered with DSB-inducing therapeutic modalities it enhances their sensitivity significantly. SCR7 to target NHEJ offers a novel strategy toward the treatment of cancer and improvement of existing regimens.
SCR7 is a potent and selective inhibitor of non-homologous end joining (NHEJ). It inhibits joining of DSBs in cell-free DNA repair system, blocks Ligase IV-mediated joining by interfering with its DNA binding but not that of T4 DNA Ligase or Ligase I, thereby leading to accumulation of DSBs within the cells, culminating into cytotoxicity. SCR7 inhibits NHEJ in a Ligase IV-dependent manner within cells, and activates the intrinsic apoptotic pathway. More importantly, SCR7 impedes tumor progression in mouse models, and when co-administered with DSB-inducing therapeutic modalities it enhances their sensitivity significantly. SCR7 to target NHEJ offers a novel strategy toward the treatment of cancer and improvement of existing regimens.
T7 High Yield RNA Transcription Kit
產品訂購資訊 : ZGVTR101-01 50rxn
產品介紹
每個反應產生高達200 μg的RNA產物 T7 High Yield Transcription Kit 是使用T7 RNA 聚合酶進行體外轉錄的優化反應，適用於體外轉錄合成大量的RNA產物，以及將修飾過的核苷酸摻入到RNA中形成生物素或者染料標記的RNA 。本試劑盒每個20 μL的反應可以從0.5 μg的對照模版中產生150-200 μg的RNA產物，轉錄合成的RNA適用於多方面的應用，如RNA結構與功能研究，RNA酶保護實驗，探針雜交，RNAi，顯微注射。
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