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免疫發光顯色
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增強型ECL化學發光檢測試劑盒
單組份TMB顯色液 Western緩衝液 |
蛋白質定量與抽取
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BCA Protein Quantification Kit
總蛋白提取試劑盒 核蛋白/胞質蛋白提取試劑盒 膜蛋白提取試劑盒 RIPA裂解液 蛋白酶/磷酸酶抑制劑混合物 HRV 3C Protease SUMO Protease |
Markers
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Prstained Protein Marker Unstained Protein Marker
Western marker 蛋白質電泳預製膠 PAGETM Precasting Gel |
Order info
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Product Name |
Description |
Product composition |
Ordering Info |
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5X Protein Loading Buffer |
5X Protein Loading Buffer is especially formulated for use in protein sample preparations for SDS-PAGE system. A protein sample is mixed with the 5X sample buffer (4:1) and is boiled (or heated) on a heating block for 2~5 minutes. |
10% SDS, 50% Glycerol, 0.05 M DTT, 0.01 M EDTA, 0.05% Bromophenol blue and 0.125 M Tris HCl, pH approx. 6.8 |
GM47-b-50, (50 ml) GM47-b-100, (100 ml) |
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5X TG Buffer (Sterile) |
TG Buffer is the electrophoresis buffer for polyacrylamide gels, which does not include SDS for protein denature in PAGE. TG Buffer is also use for transfer of Western blot. |
0.25 M Tris, 1.92 M Glycine, pH 8.3 ± 0.05 |
GB04-1,(1 L) GB04-4, (4 x 1 L) |
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10X TGS Buffer (Sterile) |
TGS Buffer is the electrophoresis buffer for polyacrylamide gels, which includes SDS for protein denature in the PAGE. |
0.25 M Tris, 1.92 M Glycine, 1% SDS, pH 8.3 ± 0.05 |
GB05-1,(1 L) GB05-4, (4 x 1 L) |
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10X TBS Buffer (Sterile) |
TBS Buffer is used in Western blot for protein detection. |
0.25 M Tris, 1.37 M Sodium chloride, 0.027 M Potassium chloride, pH 7.4 ± 0.05 |
GB08-1,(1 L) GB08-4, (4 x 1 L) |
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10X TBST Buffer (Sterile) |
TBST Buffer is used in Western blot for protein detection. |
0.25 M Tris, 1.37 M Sodium chloride, 0.027 M Potassium chloride, 1% Tween- 20 |
GB08T-1,(1 L) GB08T-4,(4 x 1 L) |
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TMB Solution |
TMB is the most popular chromogenic substrate for horseradish peroxidase (HRP). TMB is used in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). In the presence of HRP, the reaction produces a deep blue byproduct. |
TMB substrate (1 mg/ml) in DMSO, Substrate dilution buffer |
GM61, (500 ml) |
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BSA Standard |
BSA Standards are high-quality and universally accepted reference samples for generating accurate standard curves and calibration controls in total protein assays. Useful as an electrophoresis marker and standard for protein concentration reference standards in BCA, Bradford and other protein assay protocols. |
Concentration: 1 mg/ml |
GD0069-2, (2 ml) GD0069-10, (10 ml) |
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Product Name |
Description |
Product composition |
Ordering Info |
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4X Resolving Buffer |
4X Resolving Buffer is used for the preparation of gradient, continuous and discontinuous denaturating polyacrylamide gels. |
1.5 M Tris-HCl (pH 8.8), 0.4% SDS |
GPB06, (500 ml) GPB06-1, (1000 ml) |
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4X Stacking Buffer |
4X Stacking Buffer is used for the preparation of gradient, continuous and discontinuous denaturating polyacrylamide gels. |
0.5 M Tris-HCl (pH 6.8), 0.4% SDS |
GPB07, (500 ml) GPB07-1, (1000 ml) |
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Blocking Buffer |
Blocking Buffer can minimize the non-specific signal in Western blot assay, for blocking the remaining surface of nylon or PVDF membrane. It can be used as a diluent for primary and secondary antibodies. |
10 mM Tris-HCl, 0.9% NaCl, 0.1% Triton X-100, 0.25% gelatin, 0.02% SDS |
GPB04, (500 ml) |
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10X Transfer Buffer (for Western blot) |
Transfer Buffer is a commonly used Western blot buffer for the electro-transfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes. * Methanol is not supplied but is required. |
250 mM Tris-HCl, 1.92 M Glycine |
GPB05, (500 ml) GPB05-1, (1000 ml) |
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Stripping Buffer (Mild stripping) |
Stripping Buffer is used in removal of primary and secondary antibodies from a Western blot membrane. |
1.5% Glycine, 0.1% SDS, and 1% Tween-20, pH 2.2 |
GPB08, (500 ml) |
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Stripping Buffer (Harsh stripping) |
Stripping Buffer is used in removal of primary and secondary antibodies from a Western blot membrane. |
0.063 M Tris, 2% SDS, 0.8% ß-ME, pH 6.8 |
GPB09, (100 ml) |
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20X MOPS-SDS Running Buffer (just for NuPAGE) |
MOPS-SDS Running Buffer is the electrophoresis buffer for NuPAGE polyacrylamide gels, and the buffer includes SDS for denatured protein in the PAGE. |
20X (1 M MOPS, 1 M Tris Base, 2% SDS, 20 mM EDTA) |
GB32, (500 ml) |
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Product Name |
Description |
Ordering Info |
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1.5 M Tris, pH 8.8 (Sterile) |
1.5 M Tris (hydroxymethyl) HCl solution adjusted to pH 8.8. |
GPB01, (500 ml) GPB01-1, (1000 ml) |
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1 M Tris, pH 6.8 (Sterile) |
1 M Tris (hydroxymethyl) HCl solution adjusted to pH 6.8. |
GPB02, (500 ml) GPB02-1, (1000 ml) |
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0.5 M Tris, pH 6.8 (Sterile) |
0.5 M Tris (hydroxymethyl) HCl solution adjusted to pH 6.8. |
GPB03, (500 ml) GPB03-1, (1000 ml) |
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2 M Tris, pH 8.0 (Sterile) |
2 M Tris (hydroxymethyl) HCl solution adjusted to pH 8.0. |
GPB10, (500 ml) GPB10, (1000 ml) |
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1 M Tris, pH 7.2 (Sterile) |
1 M Tris (hydroxymethyl) HCl solution adjusted to pH 7.2. |
GB39, (1 L) |
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1 M Tris, pH 7.4 (Sterile) |
1M Tris (hydroxymethyl) HCl solution adjusted to pH 7.4. |
GB40, (1 L) |
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1 M Tris, pH 7.5 (Sterile) |
1M Tris (hydroxymethyl) HCl solution adjusted to pH 7.5. |
GB41, (1 L) |
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1 M Tris, pH 8.0 (Sterile) |
1M Tris (hydroxymethyl) HCl solution adjusted to pH 8.0. |
GB42, (1 L) |
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1 M Tris, pH 8.3 (Sterile) |
1M Tris (hydroxymethyl) HCl solution adjusted to pH 8.3. |
GB49, (1 L) |
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0.2 M Tris, pH 8.3 (Sterile) |
0.2 M Tris (hydroxymethyl) HCl solution adjusted to pH 8.3. |
GB51, (1 L) |
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0.2 M Tris, pH 7.0 (Sterile) |
0.2 M Tris (hydroxymethyl) HCl solution adjusted to pH 7.0. |
GB54, (1 L) |
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2 M Tris, pH 9.0 (Sterile) |
2 M Tris (hydroxymethyl) HCl solution adjusted to pH 9.0. |
GB55, (1 L) |
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Product Name |
Description |
Order Info |
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RNAfterTM |
RNAfterTM is a non-toxic reagent for storage of various animal or plant tissues, cultured cells and bacteria for RNA purification without using liquid nitrogen or -70℃ freezer. The sample can be stored in RNAfterTM reagent for a day at 37℃, 1 week at RT, one month at 4℃ and indefinitely at -20℃. The purified RNA quality is as high as stored in liquid nitrogen. This reagent can be used in genomic DNA purification from various samples. |
GM47-b-50,
(50 ml) GM47-b-100, (100 ml) |
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DEPC (0.1%) treated water |
DEPC may be used as an added precaution when autoclaving may not be sufficient to eliminate sufficient RNase for some applications. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC. DEPC treated water is suitable for use in RNA related experiments. |
GB14, (1 L) |
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Random Primer |
Random Primers are oligodeoxyribonucleotides (mostly hexamers) used to prepare labeled DNA probes from templates for filter hybridization or in situ hybridization and to prime mRNAs with or without poly(A) for cDNA synthesis. These primers are truly random and are suitable for DNA synthesis using Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates. |
GM004, (25 μg) |
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Oligo dT Primer |
The oligo dT Primer consists 18 dT residues and is designed to prime polyA+ RNA for first-stranded cDNA synthesis. |
GM005, (25 μg) |
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10X MOPS Buffer (for RNA) |
10X MOPS Buffer is intended for use at 1X as electrophoresis and gel running buffer during the separation of RNA on denaturing formaldehyde/agarose gels. Product composition: 10X (200 mM MOPS, 20 mM Sodium acetate and 10 mM EDTA) |
GB33, (500 ml) |
VE-180 垂直電泳槽 小型蛋白質電泳槽
