力鈞生物科技有限公司 ZGENEBIO BIOTECH INC.
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ZGeneBio  Urine Circulating Nucleic Acid Extraction  Kit

Cat# ZGCIR03-20   
Size : 20Reactions


For concentration and purification of Circulating DNA, RNA and micro RNA from Urine
NGS / QPCR / PCR

Introduction 
Raised  levels of cell-free circulating DNA in cancer patients have been reported in  many tumor types and analysis of circulating DNA can provide a useful marker for  earlier cancer detection. Reported circulating DNA has a quite small fragment  size which is about 200bp. Based on the high efficiency and specificity between  nucleic acid and ZGeneBio  beads. ZGeneBio  developed  the Free Criculating Nucleic Acid Extraction Kit from Urine. 

The human urine have been shown to contain  cell-free DNA. For urine DNA detection, several recent studies addressed the  need for careful
 evaluation and standardization of preanalytical  processes. Key problems appear, such as possible contamination of cells; the  generally low and variable amount of free circulating DNA, making  extraction/quantification difficult and time-consuming; poor DNA quality; and  the presence of PCR inhibitors. In any case, automation of DNA extraction, which  is a prerequisite for introduction of these diagnostic approaches in clinical
laboratories, is difficult to achieve because of the volumes of plasma necessary  to get sufficient DNA. The  ZGeneBio  Free Circulating Nucleic Acid Kit is a simple, rapid method and time-saving  process, which can be routinely used in laboratories. The  entire procedure takes less than 1 hour as many  as 12 samples can be handled in one cycle. It also enables efficient  purification of these free circulating nucleic acids and provides high yields  from human urine. The kit could starting sample volumes of up to 15 ml, and  flexible elution volumes between 100 μl and 200 μl allow concentration of  nucleic acid species that are present in low amounts in the sample material. The  kit can also be used for purification and concentration of viral nucleic acids  from large sample volumes.