ZGeneBio Urine Circulating Nucleic Acid Extraction Kit
Cat# ZGCIR03-20
Size : 20Reactions
For concentration and purification of Circulating DNA, RNA and micro RNA from Urine
NGS / QPCR / PCR
Introduction
Raised levels of cell-free circulating DNA in cancer patients have been reported in many tumor types and analysis of circulating DNA can provide a useful marker for earlier cancer detection. Reported circulating DNA has a quite small fragment size which is about 200bp. Based on the high efficiency and specificity between nucleic acid and ZGeneBio beads. ZGeneBio developed the Free Criculating Nucleic Acid Extraction Kit from Urine.
The human urine have been shown to contain cell-free DNA. For urine DNA detection, several recent studies addressed the need for careful
evaluation and standardization of preanalytical processes. Key problems appear, such as possible contamination of cells; the generally low and variable amount of free circulating DNA, making extraction/quantification difficult and time-consuming; poor DNA quality; and the presence of PCR inhibitors. In any case, automation of DNA extraction, which is a prerequisite for introduction of these diagnostic approaches in clinical
laboratories, is difficult to achieve because of the volumes of plasma necessary to get sufficient DNA. The ZGeneBio Free Circulating Nucleic Acid Kit is a simple, rapid method and time-saving process, which can be routinely used in laboratories. The entire procedure takes less than 1 hour as many as 12 samples can be handled in one cycle. It also enables efficient purification of these free circulating nucleic acids and provides high yields from human urine. The kit could starting sample volumes of up to 15 ml, and flexible elution volumes between 100 μl and 200 μl allow concentration of nucleic acid species that are present in low amounts in the sample material. The kit can also be used for purification and concentration of viral nucleic acids from large sample volumes.
Cat# ZGCIR03-20
Size : 20Reactions
For concentration and purification of Circulating DNA, RNA and micro RNA from Urine
NGS / QPCR / PCR
Introduction
Raised levels of cell-free circulating DNA in cancer patients have been reported in many tumor types and analysis of circulating DNA can provide a useful marker for earlier cancer detection. Reported circulating DNA has a quite small fragment size which is about 200bp. Based on the high efficiency and specificity between nucleic acid and ZGeneBio beads. ZGeneBio developed the Free Criculating Nucleic Acid Extraction Kit from Urine.
The human urine have been shown to contain cell-free DNA. For urine DNA detection, several recent studies addressed the need for careful
evaluation and standardization of preanalytical processes. Key problems appear, such as possible contamination of cells; the generally low and variable amount of free circulating DNA, making extraction/quantification difficult and time-consuming; poor DNA quality; and the presence of PCR inhibitors. In any case, automation of DNA extraction, which is a prerequisite for introduction of these diagnostic approaches in clinical
laboratories, is difficult to achieve because of the volumes of plasma necessary to get sufficient DNA. The ZGeneBio Free Circulating Nucleic Acid Kit is a simple, rapid method and time-saving process, which can be routinely used in laboratories. The entire procedure takes less than 1 hour as many as 12 samples can be handled in one cycle. It also enables efficient purification of these free circulating nucleic acids and provides high yields from human urine. The kit could starting sample volumes of up to 15 ml, and flexible elution volumes between 100 μl and 200 μl allow concentration of nucleic acid species that are present in low amounts in the sample material. The kit can also be used for purification and concentration of viral nucleic acids from large sample volumes.
